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Primary Cells Versus Cell Lines – ScienCell Research Laboratories |
Cell culture studies provide a valuable complement to in vivo experiments, allowing for a more controlled manipulation of cellular functions and processes. For decades, cell lines have played a critical role in scientific advancements, yet researchers have become increasingly cautious when interpreting data generated from cell lines only. Factors such as misidentified and contaminated cell lines have spurred renewed interest in primary cells [1,2]. Many researchers have chosen to work with cells lines as they are generally highly proliferative, and easier to culture and transfect. Most cell lines have been in culture for decades and are well adapted to the two-dimensional culture environment, and as a result, often differ genetically and phenotypically from their tissue origin and show altered morphology [3,4]. In contrast to cell lines, primary cells which are isolated directly from tissues, have a finite lifespan and limited expansion capacity. On the positive side, primary cells have normal cell morphology and maintain many of the important markers and functions seen in vivo [3,4]. Endothelial cell lines, for example, lack various functional markers, while primary endothelial cells retain these critical features.
cellline  primarycells  biology  wetlab  explained 
january 2019 by kme
r/K selection theory - Wikipedia, the free encyclopedia
K-selection strategy: few offspring, long gestation, long parental care, and a long period until sexual maturity.

r-selection strategy: many offspring, short gestation, less parental care, and a short time until sexual maturity.
mating  biology 
may 2016 by kme
Scientists bust myth that our bodies have more bacteria than human cells : Nature News & Comment
Putting together these kinds of calculations, the researchers produce a ratio for microbial to human cells for the average man of 1.3:1, with a wide uncertainty. Milo declined to comment on the paper, because it is in review at a scientific journal.
gut  bacteria  microbiota  symbiosis  biology  science 
may 2016 by kme

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